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Biomarkers go hand in hand with microscopy, as the mainstay of noncultivation-based procedures for taking a microbiological census in natural habitats such as water, sediment, and soil We saw in 2 (Section 21, Table 22, and Box 22) that biomarkers (especially stable isotopic ratios and molecular fossils) have proven essential for discovering clues about the ancient history of prokaryotic life The biomarkers that have proven most insightful for contemporary microorganisms are nucleic acids especially the sequences of taxonomically and evolutionarily insightful ribosomal RNA genes [ie, small subunit 16S rRNA (in prokaryotes) and 18S rDNA (in eukaryotes); see Section 55].

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running in parallel on a low-latency parallel machine and for linking disparate resources (storage, computation, visualization, instruments) The fundamental Grid task of managing these heterogeneous components as we scale the size of distributed systems replaces that of the tight synchronization of the typically identical [in program but not data as in the SPMD (single program multiple data) model] parts of a domain-decomposed parallel application During the 1980s, researchers from multiple disciplines also began to come together to attack Grand Challenge problems [17], that is, key problems in science and engineering for which large-scale computational infrastructure provided a fundamental tool to achieve new scienti c discoveries The Grand Challenge and multidisciplinary problem teams provided a model for collaboration that has had a tremendous impact on the way largescale science is conducted to date Today, interdisciplinary research has not only provided a model for collaboration but has also inspired whole disciplines (e.

/* We know the hypotenuse and the one side of the triangle, and want to find the value of the third side. */ var nHypotenuse:Number = 5; var nA:Number = 3; var nB:Number; // unknown // Given that a^2 + b^2 = hypotenuse^2 nB = Math.sqrt(Math.pow(nHypotenuse, 2) Math.pow(nA, 2));

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g bioinformatics) that integrate formerly disparate areas of science The problems inherent in conducting multidisciplinary and often geographically dispersed collaborations provided researchers experience both with coordination and distribution two fundamental concepts in Grid Computing In the 1990s, the US Gigabit testbed program [18] included a focus on distributed metropolitan-area and wide-area applications Each of the test beds Aurora, Blanca, Casa, Nectar and Vistanet was designed with dual goals: to investigate potential testbed network architectures and to explore their usefulness to end users In this second goal, each testbed provided a venue for experimenting with distributed applications The rst modern Grid is generally considered to be the information wide-area year (IWAY), developed as an experimental demonstration project for SC95 In 1995, during the week-long Supercomputing conference, pioneering researchers came together to aggregate a national distributed testbed with over 17 sites networked together by the vBNS.

Nucleic acid biomarkers have had a profound impact on environmental microbiology for three major reasons: (i) ne distinctions between nucleotide sequences can be made when many of these extracted biomarkers are compared; (ii) the sensitivity for detecting (cloning and sequencing) nucleic acid is very high due to the applicability of the polymerase chain reaction (PCR) in recovering sequences derived from environmental samples; and (iii) identical.

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