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(1983) Polarization of thyroid cells in culture; evidence for the basolateral localization of the iodide pump and of the thyroid-stimulating hormone receptor-adenyl cyclase complex J Cell Biol 96: 1172 1177 Chambard, M, Mauchamp, J, Chaband, O (1987) Synthesis and apical and basolateral secretion of thyroglobulin by thyroid cell monolayers on permeable substrate: Modulation by thyrotropin J Cell Physiol 133: 37 45 Chen, TR (1977) In situ detection of mycoplasma contamination in cell cultures by uorescent Hoechst 33258 stain Exp Cell Res 104: 255 Filla, MS, Dam, P, Rapraeger, AC (1998) The cell surface proteoglycan syndecan-1 mediates broblast growth factor-2 binding and activity J Cell Physiol 174: 310 321 Freshney, RI (2002) Cell line provenance Cytotechnology 39: 3 15 Freshney, RI (2005) Culture of Animal Cells, a Manual of Basic Technique, 5th Ed Hoboken NJ, John Wiley & Sons Greco, B.

Then construct the method to handle the cool_down mode:

, Recht, L (2003) Somatic plasticity of neural stem cells: Fact or fancy J Cell Biochem 88: 51 56 Ham, RG, McKeehan, WL (1978) Development of improved media and culture conditions for clonal growth of normal diploid cells In Vitro 14: 11 22 Hay, RJ, Miranda-Cleland, M, Durkin, S, Reid, YA (2000) Cell line preservation and authentication In Masters, JRW, ed, Animal Cell Culture, 3rd Ed Oxford, Oxford University Press, pp 69 103 Juliano, RL (2002) Signal transduction by cell adhesion receptors and the cytoskeleton: functions of integrins, cadherins, selectins, and immunoglobulin-superfamily members Annu Rev Pharmacol Toxicol 42: 283 323 Karmiol, S (2000) Development of serum-free media In Masters, JRW, ed, Animal Cell Culture, 3rd Ed Oxford, Oxford University Press, pp 105 121 Klement, G, Scheirer, W, Katinger, HW.

(1987) Construction of a large scale membrane reactor system with different compartments for cells, medium and product Dev Biol Stand, 66: 221 226 Knazek, RA, Gullino, P, Kohler, PO, Dedrick, R (1972) Cell culture on arti cial capillaries An approach to tissue growth in vitro Science 178: 65 67 Kruse, PF, Jr, Keen, LN, Whittle, WL (1970) Some distinctive characteristics of high density perfusion cultures of diverse cell types In Vitro 6: 75 78 Laning, JC, DeLuca, JE, Hardin-Young, J (1999) Effects of immunoregulatory cytokines on the immunogenic potential of the cellular components of a bilayered living skin equivalent Tissue Eng 5: 171 181 Lopez-Casillas, F, Wrana, JL, Massague, J (1993) Betaglycan presents ligand to the TGF- signalling receptor Cell 73: 1435 1444 Maas-Szabowski, N, Stark, H-J.

, Fusenig, NE (2002) Cell interaction and differentiation In Freshney RI and Freshney MG, eds, Culture of Epithelial Cells, 2nd Ed New York, John Wiley & Sons, pp 31 63 MacLeod, RA, Dirks, WG, Matsuo, Y, Kaufmann, M, Milch, H, Drexler, HG (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source Int J Cancer 83: 555 63 Marcovic, O, Marcovic, N (1998) Cell cross-contamination in cell cultures: the silent and neglected danger In Vitro Cell Dev Biol 34: 108..

is a broadcasting frame including the destination address in the MAC payload This enables devices to sense activity of the channel without ltering the MAC layer The time line of LP is divided into wakeup intervals Each wakeup interval is started by a short active duration followed by a long inactive duration The time line of each device is asynchronous to each other To transmit a data frame, a device transmits a sequence of WRs longer than a wakeup interval followed by a data frame While the transmitter transmitts WRs, other devices receive at least one WR in their active duration If the address is matched, then the device stays awake until it receives the data frame at the end of the WR sequence If the address is not matched, the device goes to sleep Figure 184 gives an example of the time line of the LP.

(17.60)

force after internal temperature reach 70 (Ritchey and Hostetler 1965; Bouton et al. 1976, 1982; Leander et al. 1980). However, other researchers have reported a decrease in shear force values at temperatures between 50 C and 60 C (Davey and Neiderer 1977; Hearne et al. 1978). The different observations could be associated with temperatures being evaluated. Bouton et al. (1976) reported

Compared to the algorithms on the beacon mode, the wakeup duration lasts only long enough to receive a short WR Since it does not require time for a beacon and a maximum-sized frame, the active duration can be minimized However, the transmitter has to transmit WRs longer than the wakeup interval tWI to transmit a data frame The receiver also has to spend energy for a half of the wakeup interval (tWI/2) on average to nally receive a data frame Therefore, the energy consumption is highly dependent on the frame arrival rate r and tWI 18322 Long Preamble Emulation with Acknowledgment LPA adopts the asynchronous algorithm X-MAC or TICER proposed in [3, 4] Based on LP, LPA introduces a new command frame named wakeup acknowledgment (WA) The WR and the WA are broadcasting frames for the same reason given for LP.